HR5 EXTRA INFO: HELA (C005B-238325)

Cell Line Origin

Human cervix carcinoma, HeLa derivative, transformed

Cell Line Description

HR5 cells were obtained after transfection of HeLa cells with plasmids coding for a reverse tetracycline-controlled transactivator encoding a nuclear localisation sequence (rt TA-nls) and transferring G418 resistance. This transactivator (a fusion between the Tet repressor and the activating domain of the herpes simplex virus VP16 transcription activation domain) can stimulate the expression of a reporter gene up to 10E5-fold. HR5 is the parent cell line for HR5-CL11 (Sigma Catalogue number.95091411).

Application

Gene expression and activation studies

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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biological source: human cervix. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

51286102

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

VI

MPN:

95091409-1VL

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes