EMSA Probe HIF-1α (C007B-555755)

Aladdin's EMSA Probe - HIF-1± is a HIF-1± consensus oligonucleotide designed for EMSA . This double-stranded oligonucleotide contains the well-recognized binding sequence for hypoxia-inducible factor 1-alpha (HIF-1±), and can be used as a probe for EMSA. The nucleotide sequence of HIF-1± consensus oligo is as followsPrecautions:Avoid heating above 40°C, as double-stranded DNA probe will be denatured at high temperatures into single strands that cannot be used in EMSA.For detailed EMSA procedures, please refer to the manual of EMSA kits from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use:1.Set up the probe labeling reaction with isotopes by adding reagents in sequence as follows:Probe to be labeled (1.75M)2lT4 Polynucleotide Kinase 10X Buffer1lNuclease-Free Water5l[³-32P]ATP (3,000Ci/mmol at 10mCi/ml)1lT4 Polynucleotide Kinase (5€“10u/l)1lTotal volume10l2.To label the probe with biotin, please referred to the EMSA Probe Biotin Labeling Kit (, GS008).3.For cold competition reaction setup, the amount of unlabeled probe should be 50-100 times that of labeled probe.

Specification: 10M

UPC:

41116006

Condition:

New

Weight:

0.07 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

E745606-30μl

Product Size:

30μl