EndoAlertT Endotoxin Plate Kit, 1Kit

The EndoAlert™ Endotoxin Plate Kit is a kinetic, colorimetric assay for the quantitative determination of bacterial Endotoxin in aqueous solutions. Endotoxin, a bacterial lipopolysaccharide, is one of the major cell wall components of most gram-negative bacteria. The EndoAlert™ Endotoxin Plate Kit detects low levels of Endotoxin and is therefore a useful tool to assess the integrity of biological and environmental samples. The detection ranges from 0.01 - 10 Endotoxin units (EU/mL). The EndoAlert™ Endotoxin Plate Kit is a quantitative version of the reaction first described by Levin and Bang in 1968. The test is based upon an enzymatic cascade where Endotoxin activates Factor C in Limulus Amebocyte Lysate (LAL) which in turn activates Factor B. Factor B activates Proclotting Enzyme which then activates Clotting Enzyme. A colorless synthetic peptide substrate is hydrolyzed by Clotting Enzyme to generate a yellow color which can be measured by a spectrophotometer at 405 nm. The degree of color resulting from the reaction is proportional to the amount of Endotoxin in the test sample and can be calculated using a standard curve. Application Note Endotoxin level detection. See kit protocol for complete details. The detection ranges from 0.01 - 10 Endotoxin units (EU/mL). Incubation 60 mins.

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UPC:

41115813

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No"

WeightUOM:

LB

MPN:

KMA-0100

Model Number:

CHM03V373

Temperature Control Device:

Yes