ERK ACTIVATION INHIBITOR PEP 1PC X 1MG (C005B-227273)

General description

A cell-permeable and reversible stearated 13-amino acid peptide corresponding to the N-terminus of MEK1 (MAPKK) that acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of Elk1. Selectively binds to ERK2 and prevents its interaction with MEK (IC₅₀ = 2.5 µM). Potently blocks ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells (IC₅₀ = 13 µM). Does not affect the activation of JNKs or p38.

A cell-permeable, reversible, and stearated, 13-amino acid peptide corresponding to the N-terminus of MAPK/ERK kinase (MEK). Acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of ELK1. Selectively binds to ERK2 and prevents its interaction with MEK (IC₅₀ = 2.5 µM). Shown to block ERK activity in PMA-stimulated NIH3T3 cells and NGF-treated PC12 cells (IC₅₀ = 13 µM). Does not affect the activation of either JNKs or p38 MAP kinase.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
MEK

Product does not compete with ATP.

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Ste-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂ (Ste = stearated)

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Kelemen, B.R., et al. 2002. J. Biol. Chem.277, 8741.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 1757.32. Empirical Formula: C₈₆H₁₅₃N₁₉O₁₇S. Quality Level: 100. Assay: ≥. 95% (HPLC). form: lyophilized solid. potency: 2.5 . μ. M IC₅₀. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, desiccated (hygroscopic), protect from light. color: white. solubility: water: 1 . mg/mL, DMSO: 5 . mg/mL. shipped in: wet ice. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

51111742

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

MG

MPN:

328000-1MG

Temperature Control Device:

Yes