Storage buffer: 50mM Tris, 50mM KCl, 1mM DTT, 0.05mM EDTA, 50% Glycerol, 200 μg/ml HSA, pH 8.0Exonuclease III has 3 '→5' exonuclease activity on double-stranded DNA, which can degrade flat end, 3 'end dented and incised DNA, and degrade DNA molecules from the 3' end. For 3 'protruding ends, especially protruding DNA molecules 4 nt or longer cannot be cut at all. In addition, the active site of the enzyme depends on the helical structure and varies according to the sequence (C>A=T>G).Product composition:rp216608Component5KU25KUStoragerp216608AExonuclease III50 μL250 μL-20°C. Avoid freeze/thaw cyclerp216608B10×Exonuclease III Reaction Buffer750 μL3.75 mL-20°C. Avoid freeze/thaw cycleSourceE.coliEnzyme Activity DefinitionThe amount of enzyme required to catalyze the production of 1 nmol acid-soluble total nucleotide in a 50 μl reaction system at 37℃ for 30 min is defined as 1 unit (U).Applications(1) Non-directional nested deletion;(2) Site-directed mutagenesis;(3) Preparation of chain specific probes;(4) Preparation of single-stranded substrates for dideoxy sequencing.Protocol(1) Configure the reaction system, as shown in the following tableComponentVolumeDNA5 μg10×Exonuclease III Reaction Buffer5 μlExonuclease III0.5 μlNuclease-free WaterUp to 50 μl(2) Incubation at 37 "C for 30min.(3) Incubation at 75°C for 10 min terminated the reaction.Cautions(1) The reaction temperature, the concentration of salt ions in the system and the ratio of enzyme to DNA will affect the activity of the enzyme.(2) This product is for scientific research only and shall not be used for other purposes.
Specifictaions: EnzymoPure™, BioReagent, for DNA synthesis, Suitable for molecular biology, PCR Reagent, For In Vitro Transcription, for DNA and RNA applications, 100 U/μl
- UPC:
- 41105317
- Condition:
- New
- Weight:
- 7.06 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- rp216608-25KU
- Product Size:
- 25KU
