General description
In contrast to Fmoc-Lys(biotin)-OH, this novel biotin-labeled amino acid has excellent solubility in DMF and other solvents used in Fmoc SPPS [1]. The PEG-spacer restricts hindrance between the peptide and avidin, leading to better biotin binding. Furthermore, the hydrophilic nature of the PEG minimizes non-specific interactions that can arise from the spacer group becoming buried in the hydrophobic pocket of proteins.
Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis
Literature references
[1] B. Baumeister, et al. (2003) Biopolymers, 71, 339.
Linkage
Replaces: 04-12-1279
Analysis Note
Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: ≥ 99.5 % (a/a)
Purity (TLC(CMA2)): ≥ 98 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (1 mmole in 2 ml DMF): clearly soluble
To see the solvent systems used for TLC of Novabiochem® products please click here.
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
Molecular Weight: 783.93. Empirical Formula: C39H53N5O10S. Quality Level: 200. product line: Novabiochem®. . Assay: ≥. 95.0% (HPLC), ≥. 98% (TLC). form: powder. reaction suitability: reaction type: Fmoc solid-phase peptide synthesis. manufacturer/tradename: Novabiochem®. . application(s): peptide synthesis. functional group: biotin. storage temp.: 2-8°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51132240
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 8521138500