FMOC-GLU(BIOTINYL-PEG)-OH 1 G

General description

In contrast to Fmoc-Lys(biotin)-OH, this novel biotin-labeled amino acid has excellent solubility in DMF and other solvents used in Fmoc SPPS ^[1]. The PEG-spacer restricts hindrance between the peptide and avidin, leading to better biotin binding. Furthermore, the hydrophilic nature of the PEG minimizes non-specific interactions that can arise from the spacer group becoming buried in the hydrophobic pocket of proteins.For recent applications, please see ^[2,3,4].

Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Biotinylation Reagents for Peptide Synthesis


Literature references

^[1] B. Baumeister, et al. (2003) Biopolymers, 71, 339.
^[2] X. Zhou et al., (2004) J. Am. Chem. Soc., 126, 15656.
^[3] B. F. Gilmore, et al. (2006) Biochem. Biophys. Res. Commun,, 347, 373.
^[4] C. T. Archer, et al. (2005) Mol. BioSyst., 1, 366.

Linkage

Replaces: 04-12-1250

Analysis Note

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: ≥ 99.5 % (a/a)
Purity (TLC(CMA2)): ≥ 97 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (0,2 mmol in 1 ml DMF): clearly soluble

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

UPC:

51322501

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

GR

MPN:

8521020001

CAS:

817169-73-6