General description
In contrast to Fmoc-Lys(biotin)-OH, this novel biotin-labeled amino acid has excellent solubility in DMF and other solvents used in Fmoc SPPS [1]. The PEG-spacer restricts hindrance between the peptide and avidin, leading to better biotin binding. Furthermore, the hydrophilic nature of the PEG minimizes non-specific interactions that can arise from the spacer group becoming buried in the hydrophobic pocket of proteins.For recent applications, please see [2,3,4].
Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Biotinylation Reagents for Peptide Synthesis
Literature references
[1] B. Baumeister, et al. (2003) Biopolymers, 71, 339.
[2] X. Zhou et al., (2004) J. Am. Chem. Soc., 126, 15656.
[3] B. F. Gilmore, et al. (2006) Biochem. Biophys. Res. Commun,, 347, 373.
[4] C. T. Archer, et al. (2005) Mol. BioSyst., 1, 366.
Linkage
Replaces: 04-12-1250
Analysis Note
Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: ≥ 99.5 % (a/a)
Purity (TLC(CMA2)): ≥ 97 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (0,2 mmol in 1 ml DMF): clearly soluble
To see the solvent systems used for TLC of Novabiochem® products please click here.
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51131802
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 8521028500