General description
A useful derivative for the synthesis of phosphotyrosine peptides [1,2,3,4,5,67] by Fmoc SPPS. Pyrophosphate formation has been noted in peptides containing adjacent Tyr(PO3H2) residues [4,5]. An evaluation of the optimal coupling conditions for introduction of this residue has been made [6].
Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Literature references
[1] E. A. Ottinger, et al. (1993) Biochemistry, 32, 4354.
[2] E. A. Ottinger, et al. (1995) Int. J. Peptide Protein Res., 46, 346.
[3] F. Anjuere, et al. (1995) Anal. Biochem., 229, 61.
[4] C. Garcia-Echeverria (1995) Lett. Pept. Sci., 2, 93.
[5] R. M. Valerio, et al. (1995) Lett. Pept. Sci., 2, 33.
[6] C. Garcia-Echeverria (1996) Lett. Pept. Sci., 2, 369.
[7] L. F. Bonewald, et al. (1999) J. Peptide Res., 53, 161.
Linkage
Replaces: 04-12-1125
Analysis Note
Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: ≥ 99.5 % (a/a)
Purity (TLC(CMA1)): ≥ 95 %
Purity (TLC(0110)): ≥ 95 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (1 mmole in 2 ml DMF): clearly soluble
Assay (acidimetric): ≥ 85.0 %
Water (K. F.): ≤ 2.0 %
Ethyl acetate (HS-GC): ≤ 0.5 %
Acetate (IC): ≤ 0.1 %
To see the solvent systems used for TLC of Novabiochem® products please click here.
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41115701
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 8520580005
