Glucose Content Assay Kit (Folin-Wu, Micro Method)

Glucose (Glucose, Dextrose, Glu), also known as corn sugar, has the chemical formula C₆H₁₂O₆ and a molecular weight of 180.16. It is one of the most widely distributed and important monosaccharides in nature, belonging to the class of polyhydroxy aldehydes. While enzymatic methods like glucose oxidase and hexokinase are common in biochemical testing, this kit is based on a different chemical principle. The detection principle of this kit is as follows: Glucose in a protein-free blood filtrate reduces copper ions in a heated alkaline environment, forming a precipitate of cuprous oxide. This cuprous oxide then reduces a molybdate reagent to a lower valence blue molybdenum compound (molybdenum blue), causing the solution to turn blue. The intensity of the blue color is proportional to the glucose concentration, with a maximum absorption peak at 640 nm. The glucose content in the sample can be calculated by measuring the absorbance of both the sample and the standard using a microplate reader and applying the appropriate formula. This kit is intended for the quantitative determination of glucose content in samples such as human or animal serum and plasma. Note that the Glucose standard (5 mmol/L) provided is equivalent to 90 mg/dL. This product is for research use only and is not intended for use in diagnostic procedures.

Specifications & Purity: Glucose (Glucose, Dextrose, Glu), also known as corn sugar, has the chemical formula C₆H₁₂O₆ and a molecular weight of 180.16. It is one of the most widely distributed and important monosaccharides in nature, belonging to the class of polyhydroxy aldehydes. While enzymatic methods like glucose oxidase and hexokinase are common in biochemical testing, this kit is based on a different chemical principle. The detection principle of this kit is as follows: Glucose in a protein-free blood filtrate reduces copper ions in a heated alkaline environment, forming a precipitate of cuprous oxide. This cuprous oxide then reduces a molybdate reagent to a lower valence blue molybdenum compound (molybdenum blue), causing the solution to turn blue. The intensity of the blue color is proportional to the glucose concentration, with a maximum absorption peak at 640 nm. The glucose content in the sample can be calculated by measuring the absorbance of both the sample and the standard using a microplate reader and applying the appropriate formula. This kit is intended for the quantitative determination of glucose content in samples such as human or animal serum and plasma. Note that the Glucose standard (5 mmol/L) provided is equivalent to 90 mg/dL. This product is for research use only and is not intended for use in diagnostic procedures.

Grade: BioReagent

Storage Temperature: Store at 2-8°C;Protected from light

Shipped in: Wet ice

UPC:

41141812

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

G1521748-100T

Country of Origin:

China

Product Size:

100T

Harmonized Tariff Code:

2931.90.90