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Sigma-Aldrich

GLYCEROL ASSAY KIT (C005B-478069)

General description Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides.

Description

General description

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Principle

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

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Specifications
UPC:
41141833
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
1
Unit of Measurement:
KT
MPN:
MAK117-1KT
Temperature Control Device:
Yes
Catalog No. C005B-478069
Price: $1,017.88
List Price: $1,130.98
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