Goat Anti-Mouse IgG (H+L)

Preparation Method: This product consists of 0.02M sodium phosphate, 0.15M NaCl, pH 7.6, 0.05% Sodium azide. It has been isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Background Information: IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. It regulates several functions such as complement activation and phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-mouse IgG (whole molecule) antibody can be used in T cell proliferation assays. It can also be used in Ouchterlony Double Diffusion and immunoelectrophoresis. Goat anti- mouse IgG (whole molecule) antibody reacts specifically with mouse IgG subclasses IgG1, IgG2a, IgG2b and IgG3. The product has also shown no reactivity to human serum proteins. Applications: IgG H&L CHAIN POLYCLONAL ANTIBODY can be used in Indirect immunofluorescence. It can also be used as a secondary antibody in western blotting to release NC1 domain of type IV collagen. Further, it can also be used in enzyme immunoassays and immunohistochemistry. Product Description: Anti-mouse IgG (whole molecule) is developed in goat using mouse IgG purified from pooled normal mouse serum as the immunogen.