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Sigma-Aldrich

HCA-7

Cell Line Origin Human colon adenocarcinoma Cell Line Description Established from a colon adenocarcinoma of a 58 year-old female patient. The adenocarcinoma was moderately differentiated and determined to be Dukes′: stage B.

Description

Cell Line Origin

Human colon adenocarcinoma

Cell Line Description

Established from a colon adenocarcinoma of a 58 year-old female patient. The adenocarcinoma was moderately differentiated and determined to be Dukes′ stage B. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,14
D13S317: 9
D16S539: 10,11
D5S818: 9,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,10
vWA: 14,16

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:10 using 0.05% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

Specifications
UPC:
51281657
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
1
Unit of Measurement:
VL
MPN:
06061902-1VL
Temperature Control Device:
Yes
Catalog No. C005B-244427
Price: $1,906.09
List Price: $2,117.88
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