Hemoglobin Content Assay Kit (Hematin, Micro Method)

Hemoglobin (Hb or HGB) is a protein responsible for transporting oxygen in higher organisms and is the protein that gives blood its red color. Hemoglobin is composed of four chains: two α chains and two β chains, each containing a heme group with an iron atom. Hemoglobin readily binds to oxygen in areas with high oxygen concentration and easily dissociates from it in areas with low oxygen concentration. This property of hemoglobin enables red blood cells to transport oxygen. Currently, the primary methods for hemoglobin detection include the cyanmethemoglobin oxidation method, alkaline hematin determination, sodium lauryl sulfate binding method, and copper sulfate titration method. Alternatively, imported large-scale biochemical analyzers are used for measurement. The cyanmethemoglobin oxidation method involves the toxicity and hazardous waste issues associated with potassium cyanide. The copper sulfate titration method is prone to preparation errors and is easily affected by environmental temperature. Biochemical analyzers, on the other hand, are expensive and have high testing costs. The detection principle of this kit is based on the oxidation of ferrous ions (Fe²⁺) in hemoglobin to ferric ions (Fe³⁺) by potassium ferricyanide, converting hemoglobin into methemoglobin. Methemoglobin then reacts with azide salt to form stable azide methemoglobin, which has a broad absorption peak at 540–546 nm. The absorbance is directly proportional to the hemoglobin concentration. This product is used to measure hemoglobin content in blood, aiding in the diagnosis of conditions such as anemia and blood loss. This product is intended for research purposes only and is not suitable for clinical diagnosis or other uses.

Specifications & Purity: Hemoglobin (Hb or HGB) is a protein responsible for transporting oxygen in higher organisms and is the protein that gives blood its red color. Hemoglobin is composed of four chains: two α chains and two β chains, each containing a heme group with an iron atom. Hemoglobin readily binds to oxygen in areas with high oxygen concentration and easily dissociates from it in areas with low oxygen concentration. This property of hemoglobin enables red blood cells to transport oxygen. Currently, the primary methods for hemoglobin detection include the cyanmethemoglobin oxidation method, alkaline hematin determination, sodium lauryl sulfate binding method, and copper sulfate titration method. Alternatively, imported large-scale biochemical analyzers are used for measurement. The cyanmethemoglobin oxidation method involves the toxicity and hazardous waste issues associated with potassium cyanide. The copper sulfate titration method is prone to preparation errors and is easily affected by environmental temperature. Biochemical analyzers, on the other hand, are expensive and have high testing costs. The detection principle of this kit is based on the oxidation of ferrous ions (Fe²⁺) in hemoglobin to ferric ions (Fe³⁺) by potassium ferricyanide, converting hemoglobin into methemoglobin. Methemoglobin then reacts with azide salt to form stable azide methemoglobin, which has a broad absorption peak at 540–546 nm. The absorbance is directly proportional to the hemoglobin concentration. This product is used to measure hemoglobin content in blood, aiding in the diagnosis of conditions such as anemia and blood loss. This product is intended for research purposes only and is not suitable for clinical diagnosis or other uses.

Grade: BioReagent

Storage Temperature: Store at 2-8°C;Protected from light

Shipped in: Wet ice

UPC:

41131715

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

H1522001-100T

Country of Origin:

China

Product Size:

100T

Harmonized Tariff Code:

2931.90.90