HIS-SELECT(R) FILTER PLATE

General description

The HIS-Select^® Filter Plate is an immobilized metal-ion affinity chromatography (IMAC) product that allows rapid purification of small-scale crude cell extracts containing histidine-tagged proteins. The HIS-Select^® Filter Plate contains 20 μm, spherical silica particles (100 nm pore size) with a hydrophilic layer.

The HIS-Select^® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select^® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.

Application

The HIS-Select^® Filter Plate is suitable for purification of recombinant proteins with histidine tags.

Features and Benefits

• High Selectivity for his-tagged proteins
• Ready-to-use plate format
• Compatible with CelLytic^™ cell lysis reagents

Legal Information

Covered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.

CelLytic is a trademark of Sigma-Aldrich Co. LLC

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

UPC:

41105312

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

5

Unit of Measurement:

EA

MPN:

H0413-5X1EA