Cell Line Origin
Rat hepatoma
Cell Line Description
Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.
Application
Tumourigenicity and enzymatic studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: rat liver. growth mode: Adherent. karyotype: 2n : 66, hypertriploid. morphology: Epithelial. products: Not specified. receptors: Dexamethasone phosphate. hydrocortisone. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51151643
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93120108-1VL