Human Complement Factor D ELISA Kit

This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human Complement Factor D concentration in samples. The Human Complement Factor D capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human Complement Factor D present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human Complement Factor D is added. This antibody binds to the captured Human Complement Factor D, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human Complement Factor D is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman Complement Factor D is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman Complement Factor D, and the concentration of Human Complement Factor D in unknown samples is interpolated from this curve based on their OD values.

Specifications & Purity: This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human Complement Factor D concentration in samples. The Human Complement Factor D capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human Complement Factor D present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human Complement Factor D is added. This antibody binds to the captured Human Complement Factor D, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human Complement Factor D is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman Complement Factor D is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman Complement Factor D, and the concentration of Human Complement Factor D in unknown samples is interpolated from this curve based on their OD values.

Grade: BioReagent

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

41181602

Weight:

7.06 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

H1510017-96T

Country of Origin:

China

Product Size:

96T

Harmonized Tariff Code:

2931.90.90