Human Dendritic Cell Cytokine Package (C008B-268451)

The Human Dendritic Cell Cytokine Package contains IL-4 and GM-CSF, key components required for the culturing of murine dendritic cells. Recombinant murine IL-4 is an essential cytokine used in the differentiation and maturation of murine dendritic cell in vitro. The IL-4 protein, specifically recombinant IL-4, promotes the development of dendritic cells from precursor cells such as monocytes. Murine IL-4 acts synergistically with other factors, including GM-CSF (granulocyte-macrophage colony stimulating factor), to drive the differentiation process. Typically, monocytes are cultured in the presence of recombinant IL-4 and GM-CSF to yield immature dendritic cells, which can then be futher matured by additional stimuli.The package contains the following twovials:RecombinantHuman IL-4Protein（Cat. No.rp156008）Animal-FreeRecombinantHumanGM-CSF Protein（Cat. No.rp186551）IL-4 is a pleiotropic cytokine that regulates diverse T- and B-cell responses including cell proliferation, survival, and gene expression. Produced by mast cells, T cells, and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cellsGM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells.InformationNameSpeciesExpression SystemTagPurityActivityEndotoxinAppearanceStorageReconstitutionRecombinantHumanIL-4 ProteinHumanE. coliN-His≥95% (SDS-PAGE)Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL.<1.0 EU/μgLyophilizedLyophilized from 10mM PBS, pH 7.4We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.Animal-FreeRecombinantHumanGM-CSF ProteinHuman293FNo tag≥95% (SDS-PAGE)Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 90.7 pg/mL.<0.1 EU/μgLyophilizedLyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.Notes:1. This product is for research use only.2. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications & Purity: The Human Dendritic Cell Cytokine Package contains IL-4 and GM-CSF, key components required for the culturing of murine dendritic cells. Recombinant murine IL-4 is an essential cytokine used in the differentiation and maturation of murine dendritic cell in vitro. The IL-4 protein, specifically recombinant IL-4, promotes the development of dendritic cells from precursor cells such as monocytes. Murine IL-4 acts synergistically with other factors, including GM-CSF (granulocyte-macrophage colony stimulating factor), to drive the differentiation process. Typically, monocytes are cultured in the presence of recombinant IL-4 and GM-CSF to yield immature dendritic cells, which can then be futher matured by additional stimuli.The package contains the following twovials:RecombinantHuman IL-4Protein（Cat. No.rp156008）Animal-FreeRecombinantHumanGM-CSF Protein（Cat. No.rp186551）IL-4 is a pleiotropic cytokine that regulates diverse T- and B-cell responses including cell proliferation, survival, and gene expression. Produced by mast cells, T cells, and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cellsGM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells.InformationNameSpeciesExpression SystemTagPurityActivityEndotoxinAppearanceStorageReconstitutionRecombinantHumanIL-4 ProteinHumanE. coliN-His≥95% (SDS-PAGE)Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL.<1.0 EU/μgLyophilizedLyophilized from 10mM PBS, pH 7.4We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.Animal-FreeRecombinantHumanGM-CSF ProteinHuman293FNo tag≥95% (SDS-PAGE)Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 90.7 pg/mL.<0.1 EU/μgLyophilizedLyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.Notes:1. This product is for research use only.2. For your safety and health, please wear a lab coat and disposable gloves during operation.

Grade: Sterile-filtered;BioReagent

Storage Temperature: Store at -20°C;Avoid repeated freezing and thawing

Shipped in: Ice chest + Ice pads

UPC:

51201631

Weight:

7.06 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

H1511079-10μg

Country of Origin:

China

Product Size:

10μg

Harmonized Tariff Code:

2931.90.90