HUMAN LYMPHOBLASTOID (C005B-238582)

Cell Line Origin

Human Caucasian lymphoblastoid, Cockayne syndrome, EBV-transformed

Cell Line Description

Derived from mononuclear cells from the peripheral blood of a 20 year old female. The patient has been diagnosed with Cockayne syndrome complementation group CSB. This multisystem sun-sensitive genetic disorder is associated with a DNA-repair defect after UV irradiation. The cell line LB242 has been established by EBV transformation. Fibroblasts from the same individual are designated CS4BR.

Application

DNA repair deficiency, mutation analysis

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Other Notes

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biological source: human blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

41106514

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

VI

MPN:

98062324-1VL

Temperature Control Device:

Yes