Cell Line Origin
Human lymphoblastoid, Xeroderma pigmentosum heterozygote, EBV-transformed
Cell Line Description
Established by EBV transformation of mononuclear cells from a peripheral blood sample from a 37 year old female patient. The patient was shown to be heterozygote for Xeroderma pigmentosum complementation group G, a rare, autosomal recessive disease, and is the mother of two children with XP (cell line LB83, Sigma Catalogue number. 98062328 and LB82).
Application
Toxicity studies, hprt mutation analysis
Culture Medium
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form free floating aggregates.
Other Notes
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biological source: human unknown/unspecified. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 98062327-1VL