HUMAN LYMPHOBLASTOID (C005B-238593)

Cell Line Origin

Human lymphoblastoid, Xeroderma pigmentosum, EBV-transformed

Cell Line Description

Established by EBV transformation of mononuclear cells from a peripheral blood sample from a one year old male patient. The patient was diagnosed with Xeroderma pigmentosum complementation group A, a rare autosomal recessive disease with DNA repair deficiency. A cell line established from the father of this patient is available (LB707, Sigma Catalogue number. 98062336).

Application

Toxicity studies, hprt mutation analysis

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Other Notes

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biological source: human blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

41106514

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

VI

MPN:

98062337-1VL

Temperature Control Device:

Yes