β-Hydroxybutyrate Content Assay Kit (WST-8, Micro Method)

β-Hydroxybutyrate plays an important role in the diagnosis and treatment of diabetic ketoacidosis and is also of great significance for the early diagnosis of diabetes. Under the catalysis of β-hydroxybutyrate dehydrogenase, β-hydroxybutyrate is converted into acetoacetate. At the same time, oxidized coenzyme I (NAD⁺) is reduced to reduced coenzyme I (NADH). NADH then reacts with the WST‑8 chromogenic agent to form a yellow product with a characteristic absorption peak at 450 nm. The β-hydroxybutyrate content in the sample can be calculated by measuring the increase in this yellow product at 450 nm.

Specifications & Purity: β-Hydroxybutyrate plays an important role in the diagnosis and treatment of diabetic ketoacidosis and is also of great significance for the early diagnosis of diabetes. Under the catalysis of β-hydroxybutyrate dehydrogenase, β-hydroxybutyrate is converted into acetoacetate. At the same time, oxidized coenzyme I (NAD⁺) is reduced to reduced coenzyme I (NADH). NADH then reacts with the WST‑8 chromogenic agent to form a yellow product with a characteristic absorption peak at 450 nm. The β-hydroxybutyrate content in the sample can be calculated by measuring the increase in this yellow product at 450 nm.

Grade: BioReagent

Storage Temperature: Store at 2-8°C;Protected from light

Shipped in: Wet ice

UPC:

41181801

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

H1522206-48T

Country of Origin:

China

Product Size:

48T

Harmonized Tariff Code:

2931.90.90