IdeS Protease (MS)

IdeS (IgG-specific degrading enzyme) is an engineered protease recombinantly expressed in Escherichia coli. It specifically cleaves immunoglobulin G at a single recognition site below the hinge region to generate homogeneous F(ab´)₂ and Fc fragments. Both IdeS and IdeZ proteases efficiently cleave human IgG1, IgG2, IgG3, IgG4, monkey, sheep, rabbit, humanized and chimeric IgG antibodies, as well as Fc fusion proteins. However, only IdeZ protease cleaves mouse IgG2a and IgG3. Reconstitution: Reconstitute with 40 μL of ultrapure water. Digestion Buffer: 10× buffer containing 50 mM PBS, 150 mM NaCl (pH 6.6). pH Range: Optimal activity at pH 6–8. Application: · IdeS protease is an IgG-specific protease that generates homogeneous F(ab´)₂ and Fc fragments. · Recommended Digestion SOP: · Take 10 μL of mAb sample at 10 μg/μL; · Add 5 μL of 10× digestion buffer and 31 μL of ultrapure water, mix to a total reaction volume of 50 μL; · Add 4 μL of rIdeS protease at 0.5 μg/μL; · Incubate at 37 °C for 30 minutes.

Specifications & Purity: IdeS (IgG-specific degrading enzyme) is an engineered protease recombinantly expressed in Escherichia coli. It specifically cleaves immunoglobulin G at a single recognition site below the hinge region to generate homogeneous F(ab´)₂ and Fc fragments. Both IdeS and IdeZ proteases efficiently cleave human IgG1, IgG2, IgG3, IgG4, monkey, sheep, rabbit, humanized and chimeric IgG antibodies, as well as Fc fusion proteins. However, only IdeZ protease cleaves mouse IgG2a and IgG3. Reconstitution: Reconstitute with 40 μL of ultrapure water. Digestion Buffer: 10× buffer containing 50 mM PBS, 150 mM NaCl (pH 6.6). pH Range: Optimal activity at pH 6–8. Application: · IdeS protease is an IgG-specific protease that generates homogeneous F(ab´)₂ and Fc fragments. · Recommended Digestion SOP: · Take 10 μL of mAb sample at 10 μg/μL; · Add 5 μL of 10× digestion buffer and 31 μL of ultrapure water, mix to a total reaction volume of 50 μL; · Add 4 μL of rIdeS protease at 0.5 μg/μL; · Incubate at 37 °C for 30 minutes.

Grade: Recombinant;Suitable for mass spectrometry (MS);EnzymoPure™

Storage Temperature: Store at -20°C;Avoid repeated freezing and thawing

Shipped in: Ice chest + Ice pads

UPC:

51112104

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

I1523249-20μg

Country of Origin:

China

Product Size:

20μg

Harmonized Tariff Code:

2931.90.90