General description
Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits.
Application
Invertase from baker′s yeast (S. cerevisiae) has been used:
- in liquid media (YES) and is used to determine the invertase activity
- supplemented to the reaction mixture in the extraction and analysis of sugars and starch
- for sucrose hydrolysis
Used in the production of confectionary foods and artificial honey.
Biochem/physiol Actions
Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.
Unit Definition
One unit will hydrolyze 1.0 μmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C.
- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- I9274-100MG