Isocitrate Lyase Activity Assay Kit (UV Micro Method)

ICL (EC 4.1.3.1) is mainly found in plants and microorganisms. During the germination of oilseed crops, fats are converted into carbohydrates through the glyoxylate cycle and other processes. ICL is one of the key enzymes in the glyoxylate cycle. Determination Principle: ICL catalyzes the degradation of isocitrate to glyoxylate and succinate. Glyoxylate and NADH are then converted to ethanol and NAD⁺ in the presence of LDH. NADH exhibits a characteristic absorption peak at 340 nm, and the ICL activity can be indirectly determined by monitoring the rate of decrease in absorbance at 340 nm.

Specifications & Purity: ICL (EC 4.1.3.1) is mainly found in plants and microorganisms. During the germination of oilseed crops, fats are converted into carbohydrates through the glyoxylate cycle and other processes. ICL is one of the key enzymes in the glyoxylate cycle. Determination Principle: ICL catalyzes the degradation of isocitrate to glyoxylate and succinate. Glyoxylate and NADH are then converted to ethanol and NAD⁺ in the presence of LDH. NADH exhibits a characteristic absorption peak at 340 nm, and the ICL activity can be indirectly determined by monitoring the rate of decrease in absorbance at 340 nm.

Grade: BioReagent

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

51172906

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

I1522140-100T

Country of Origin:

China

Product Size:

100T

Harmonized Tariff Code:

2931.90.90