Cell Line Origin
Human T cell, mutant derivative of Jurkat
Cell Line Description
The T cell leukaemia line J.CaM1.6 was derived from the parent cell line Jurkat E.6-1 (Sigma Catalogue number. 88042803). Jurkat cells were mutagenised with ethyl methanesulfonate and subsequently grown in phytohemagglutinin containing media for 2 weeks. J.CaM1.6 express a structurally normal but functionally defective CD3/Ti complex. The cells fail to exhibit inositolphospholipid metabolism or Ca2+ mobilisation in response to anti-CD3 or anti-Ti monoclonal antibodies. They display resistance to anti-CD3 induced apoptosis, constitutively express Fas and are sensitive to anti-Fas induced apoptosis. It was shown that J.CaM1.6 cells are defective in early signalling events that follow TCR stimulation owing to loss of lck tyrosine kinase function due to the lack of exon 7 in the lck transcript.
Application
Study of apoptosis, signal transduction receptor analysis.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 10,11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18,19
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
To keep the cells in exponential growth, maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. T
Other Notes
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biological source: human blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 96060401-1VL
