KARPAS 422

Cell Line Origin

Human B cell non-Hodgkin lymphoma

Cell Line Description

Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).

DNA Profile

Not specified

Culture Medium

RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO₂; 37 °C. Doubling time approximately 60-90 hours. Split saturated culture 1:2 every 2-4 days. Round polygonal cells growing singly or in small clusters in suspension.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Shipping Information:

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UPC:

41106514

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VL

MPN:

06101702-1VL

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes