KOSE (C005B-235302)

General description

Consanguineous: No

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 201
Cw: 1203
B: 3503
HLA DRA: 0101 0102
HLA DRB1: 1302 1401
HLA DRB3: 0301 0202
HLA DQAP: 1.3 1.4
HLA DQA1: 0104 0102
HLA DQB1: 0503 0604
HLA DPA1: 01 0201
HLA DPB1: 02012 1301

Serological Profile

HLA A: 2
HLA A ief: 2.2
HLA B: 35
HLA B ief: 35.3
Bw4/bw6: 6
HLA Dw: 9 19
HLA DR: 13 14
HLA Dw2: 25 26
HLA DR52: 52
HLA DQ: 5 6
HLA DP: 2

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source: human blood. ethnicity: German. gender: female. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. technique(s): cell culture | mammalian: suitable. shipped in: dry ice.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

41181519

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

VI

MPN:

88052056-1VL

Temperature Control Device:

Yes