L-Luciferin, potassium salt, 10mg

Tested and Certified for in vivo imaging. Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.  Luciferin is a common reagent used throughout the Biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells, or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of Luciferin allows for the real-time, non-invasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).  Luciferin is also commonly used for in vitro research; including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.  L-Luciferin is the chiralic sister to D-Luciferin. While most researchers claim that L-Luciferin does not produce any light, Lembert has reported that it produces a very weak and extremely slow manifesting light (Lembert, 1996). Nakamura suggested that an enzyme system incoporating luciferase might be responsible for changing the chirality of the L-Luciferin to D-Luciferin. Specifications: L-Luciferin, Potassium Salt 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt Forms a yellow, clear solution when dissolved 1% in water. Color: Light yellow powder  Purity: >98% by HPLC Storage/Handling: Store dessicated in -20°C and protect from light. Features CAS#: Not Available Molecular Formula:  C₁₁H₇N₂O₃S₂K Molecular Weight: 318.420 g/mol

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UPC:

51441603

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No"

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

RC-693

Model Number:

C753M96

Temperature Control Device:

Yes