Pre-mixed Gel Solutions

This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: GST--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis.

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and

Application Reducing agent &#946:-mercaptoethanol or 2-mercaptoethanol has been used: as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line) to lyse NuLi-1 (Normal Lung, University of

Application Reducing agent &#946:-mercaptoethanol or 2-mercaptoethanol has been used: as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line) to lyse NuLi-1 (Normal Lung, University of

Application Reducing agent &#946:-mercaptoethanol or 2-mercaptoethanol has been used: as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line) to lyse NuLi-1 (Normal Lung, University of

Application Reducing agent &#946:-mercaptoethanol or 2-mercaptoethanol has been used: as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line) to lyse NuLi-1 (Normal Lung, University of

2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis.

Protein samples loaded on iso-electric focusing (IEF) gels should ideally have low conductivity and be free from agents known to interfere with net protein charge. Protein samples containing interfering agents, including ionic detergents, metal

A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize proteins effectively, however can modify the native

Zwitterionic, both positively and negatively charged. Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis.Application：CHAPS is a

Zwitterionic, both positively and negatively charged. Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis.Application：CHAPS is a