LB182 HUMAN LYMPHOBLASTOID NORMAL EBV-TRANSFORMED (C005B-238576)

Cell Line Origin

Human lymphoblastoid, normal, EBV-transformed

Cell Line Description

Derived from mononuclear cells from peripheral blood of a 51 year old normal male. Cells were subsequently transformed with EBV. Fibroblasts designated 149BR (Sigma Catalogue number. 90011807) have been isolated from the same individual . This has been confirmed by STR profiling at ECACC. T cells from the same sample have been used for cellular radiosensitivity studies and cell survival analysis after UV-irradiation.

Application

Mutation studies, Toxicity analysis

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Other Notes

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biological source: human blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

41106514

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

VI

MPN:

98062317-1VL

Temperature Control Device:

Yes