LC pH Gradient Buffer B

pH gradient buffer is an innovative elution buffer (or mobile phase) that changes pH linearly for separation and purification of mAb antibodies, glycoproteins and PTM modified proteins by ion exchange (IEX) chromatography. In pH gradient buffer-based IEX chromatography, the pH of the initial buffer is kept at a constant level to ensure that the charged proteins bind to oppositely charged ions of the stationary phase. Then, the protein is eluted by linearly changing the pH of the buffer so that the protein has a zero net charge and can elute from the column. Reagent Composition: Each bottle contains 2.5 mg BT Surfactant, 850 mg NaCl, and 6 g sulfonate. Resuspension: Dissolve reagents in 500 mL deionized, double-distilled water. pH Adjustment: At room temperature, use pH meter to ensure the pH error is less than 0.2, and use 2 M NaOH or hydrochloric acid to adjust the pH. Elution Buffer (mAb): pH Buffer A, pH 5.5 Elution Buffer B (mAb): pH Buffer B, pH 10.2 Linear pH Range: Linear correlation coefficient > 0.99 at pH 5.5 - 10.2 in columns of IEX chromatography. Reference Procedure of mAb subunit and intact protein: Column: SCX, 10 μm, 2.1 * 250 mm or 2.1 * 50 mm Flow rate: 0.3 mL/min Eluent A: 1×mAb pH buffer A (pH 5.5) Eluent B: 1× mAb pH Buffer B (pH 10.2) Gradient: 0 - 1 min, 0% B; 1 - 31 min, 0 - 100% B; 31 - 34 min, 100% B; 34 - 40 min, 0% B Temperature: 30 °C FLR detection: Ex: 280 nm, Em: 360 nm UV detection: 280 nm Load: 0.2 - 10 μg Inject volume: 4 μL

Specifications & Purity: pH gradient buffer is an innovative elution buffer (or mobile phase) that changes pH linearly for separation and purification of mAb antibodies, glycoproteins and PTM modified proteins by ion exchange (IEX) chromatography. In pH gradient buffer-based IEX chromatography, the pH of the initial buffer is kept at a constant level to ensure that the charged proteins bind to oppositely charged ions of the stationary phase. Then, the protein is eluted by linearly changing the pH of the buffer so that the protein has a zero net charge and can elute from the column. Reagent Composition: Each bottle contains 2.5 mg BT Surfactant, 850 mg NaCl, and 6 g sulfonate. Resuspension: Dissolve reagents in 500 mL deionized, double-distilled water. pH Adjustment: At room temperature, use pH meter to ensure the pH error is less than 0.2, and use 2 M NaOH or hydrochloric acid to adjust the pH. Elution Buffer (mAb): pH Buffer A, pH 5.5 Elution Buffer B (mAb): pH Buffer B, pH 10.2 Linear pH Range: Linear correlation coefficient > 0.99 at pH 5.5 - 10.2 in columns of IEX chromatography. Reference Procedure of mAb subunit and intact protein: Column: SCX, 10 μm, 2.1 * 250 mm or 2.1 * 50 mm Flow rate: 0.3 mL/min Eluent A: 1×mAb pH buffer A (pH 5.5) Eluent B: 1× mAb pH Buffer B (pH 10.2) Gradient: 0 - 1 min, 0% B; 1 - 31 min, 0 - 100% B; 31 - 34 min, 100% B; 34 - 40 min, 0% B Temperature: 30 °C FLR detection: Ex: 280 nm, Em: 360 nm UV detection: 280 nm Load: 0.2 - 10 μg Inject volume: 4 μL

Grade: BioReagent

Storage Temperature: Store at -20°C

Shipped in: Ice chest + Ice pads

UPC:

41115700

Weight:

2.88 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

L1523265-6.65g

Country of Origin:

China

Product Size:

6.65g

Harmonized Tariff Code:

2931.90.90