Aladdin Scientific
Lectin from Arachis hypogaea (peanut) (C007B-556677)
General Western Blot Protocol:Glycoprotein sample size: 500ngLectin Concentration: 0.1ug/mlLoad samples at 500 ng of glycoprotein per laneRun 4-20% Bis-Tris SDS page gelTransfer gel to a PVDF membraneBlock membrane for 1 hr at RT with RIPA buffer
Description
General Western Blot Protocol:Glycoprotein sample size: 500ngLectin Concentration: 0.1ug/mlLoad samples at 500 ng of glycoprotein per laneRun 4-20% Bis-Tris SDS page gelTransfer gel to a PVDF membraneBlock membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RTWash membrane 5 x 5 minutes with 25ml RIPA bufferDetect using chemiluminescent substrate (CPS1-120)
Specification: peroxidase conjugate, lyophilized powder
Specifications
- UPC:
- 41116111
- Condition:
- New
- Weight:
- 0.07 Ounces
- HazmatClass:
- No
- Quantity:
- 1
- Unit of Measurement:
- EA
- WeightUOM:
- LB
- MPN:
- L755174-250μg
- Product Size:
- 250μg
Catalog No.
C007B-556677
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