LECTIN FROM PHASEOLUS VULGARIS (RED KIDNEY BEAN) PHYTOHEMAGGLUTININ PHA-M BIOREAGENT LYOPHILIZED POWDER CELL CULTURE TESTED (C005B-455834)

Application

Lectin from Phaseolus vulgaris (red kidney bean) is used in Lymphocyte Transformation Assay and culture medium.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

UPC:

41181705

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

100

Unit of Measurement:

MG

MPN:

L8902-100MG