Lys-C (MS)

MS-grade Lys-C protease specifically cleaves peptide bonds at the C-terminus of lysine residues. It can improve the digestion of hard-to-hydrolyze proteins and is recommended for use in 6–8 M urea solutions. Reconstitution Solution: Reconstitute Lys-C protease in 50 mM acetic acid buffer. pH Range: rLys-C exhibits optimal activity at pH 8–9. Application: Lys-C protease specifically cleaves lysine (K) residues in denatured proteins that are resistant to proteolysis. It is recommended for use in combination with trypsin. Recommended SOP for Mixed Protease Digestion: A. General In-Solution Digestion Use a mixture of 100 μg trypsin and 20 μg Lys-C at a protein-to-protease ratio (w/w) of 25:1. Incubate overnight at 37°C for 13–16 hours. Tip: Urea concentration should be <1 M; use 50 mM Tris-HCl buffer (pH 8). B. 1+1 Step In-Solution Digestion 1. Use a mixture of 100 μg trypsin and 20 μg Lys-C at a protein-to-protease ratio (w/w) of 25:1. Incubate at 37°C for 2–4 hours. Tip: Urea concentration should be 6–8 M; use 50 mM Tris-HCl buffer (pH 8). 2. Dilute the solution 6-fold and incubate overnight at 37°C for 13–16 hours. Tip: Urea concentration should be <1 M; use 50 mM Tris-HCl buffer (pH 8).

Specifications & Purity: MS-grade Lys-C protease specifically cleaves peptide bonds at the C-terminus of lysine residues. It can improve the digestion of hard-to-hydrolyze proteins and is recommended for use in 6–8 M urea solutions. Reconstitution Solution: Reconstitute Lys-C protease in 50 mM acetic acid buffer. pH Range: rLys-C exhibits optimal activity at pH 8–9. Application: Lys-C protease specifically cleaves lysine (K) residues in denatured proteins that are resistant to proteolysis. It is recommended for use in combination with trypsin. Recommended SOP for Mixed Protease Digestion: A. General In-Solution Digestion Use a mixture of 100 μg trypsin and 20 μg Lys-C at a protein-to-protease ratio (w/w) of 25:1. Incubate overnight at 37°C for 13–16 hours. Tip: Urea concentration should be <1 M; use 50 mM Tris-HCl buffer (pH 8). B. 1+1 Step In-Solution Digestion 1. Use a mixture of 100 μg trypsin and 20 μg Lys-C at a protein-to-protease ratio (w/w) of 25:1. Incubate at 37°C for 2–4 hours. Tip: Urea concentration should be 6–8 M; use 50 mM Tris-HCl buffer (pH 8). 2. Dilute the solution 6-fold and incubate overnight at 37°C for 13–16 hours. Tip: Urea concentration should be <1 M; use 50 mM Tris-HCl buffer (pH 8).

Grade: Recombinant;Suitable for mass spectrometry (MS);EnzymoPure™

Storage Temperature: Store at -20°C;Avoid repeated freezing and thawing

Shipped in: Ice chest + Ice pads

UPC:

51284505

Weight:

1.09 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

L1523246-20μg

Country of Origin:

China

CAS:

72561-05-8‌

Product Size:

20μg

Harmonized Tariff Code:

2931.90.90