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Sigma-Aldrich

M-AMINOPHENYLBORONIC ACID AGAROSE (C005B-239305)

m -Aminophenylboronic acid ( m -APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions. m -APBA is a boronate affinity matrix that is effectively used for affinity purification of

Description

General description

m-Aminophenylboronic acid (m-APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions. m-APBA is a boronate affinity matrix that is effectively used for affinity purification of monoclonal antibodies.

Application

m-Aminophenylboronic acid-Agarose has been used:

  • as a component of the column for the purification of Escherichia coli TOP10F cells
  • with cell lysates of various cells for detection of PARylated (Poly(ADP-ribos)ylation) proteins.
  • in the affinity purification of horseradish peroxidase (HRPO)
  • in the glycated human serum albumin (gHSA) purification

Physical form

Suspension in water containing 0.002% chlorhexidine diacetate.

form: aqueous suspension. Quality Level: 300. extent of labeling: 40-80 . μ. mol per mL. matrix: 6% . beaded agarose. matrix activation: epichlorohydrin. matrix attachment: through amino to carboxyls of EDTA. matrix spacer: 9 atoms. capacity: ≥. 8 . mg/mL binding capacity (peroxidase Type VI). storage temp.: 2-8°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.
Specifications
UPC:
41106510
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
5
Unit of Measurement:
ML
MPN:
A8312-5ML
Catalog No. C005B-239305
Price: $561.33
List Price: $623.70
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