Cell Line Origin
Human megakaryoblastic leukaemia
Cell Line Description
Derived from the bone marrow of a 55 year old male with chronic myelogenous leukaemia. All karyotypes show the Philadelphia chromosome. Platelet GP IIb/IIIa antigen is expressed on more than 92% of cells. Cytoplasmic expression of GP Ib antigen was not detected on the surface of cells but was present in the cytoplasm 20% of cells. BA-1 antigen is expressed in almost all cells. FVIII-related antigen was not detected on the surface cells but was present in cytoplasm.
Application
Studies of physiological mechanisms governing the differentiation of human megakaryocyte and platelet release; the study of the biochemistry (and differentiation) of erythroid and myeloid cells.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 8
D16S539: 9
D5S818: 13
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Subcultures are prepared by diluting the cell suspension into fresh medium at a ratio of 1:2 to 1:3. Cells should be seeded at 2-4 x 105 cells/ml. Doubling time is 36-48 hours. 5% CO2; 37°C.
Other Notes
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biological source: human bone marrow. growth mode: Suspension. karyotype: Modal no. 56-58, hyperdiploid. morphology: Myoblast-like. products: Immunoglobulin G (IgG)-Fc in 12% of cells. C3′. in 53% of cells. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94012401-1VL
