MM537

Cell Line Origin

Human melanoma; from metastatic site - lymph node

Cell Line Description

mM537 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1356.

DNA Profile

Not specified

Culture Medium

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.

Subculture Routine

Optimal split ratio 1:4 (minimum seeding density 2.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 1-2 min. MM537 required growth medium to be changed 3 times each week. The cells are sensitive to trypsin so exposure time to trypsin should be kept to a minimum. The cells take 2-3 days to recover from trypsinisation.

Other Notes

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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UPC:

41106514

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VI

MPN:

12070509-1VL

Temperature Control Device:

Yes