Molecular Biology

The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic, and are contaminants in Protein A isolated from S. aureus .

The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic, and are contaminants in Protein A isolated from S. aureus .

Protein A Sepharose &#8482 CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures. Protein A binds to the Fc region of immunoglobulins through interactions

A cell-permeable, symmetrical sulfonated urea compound that acts as a potent, specific and non-AdoMet (S-adenosyl-L-methionine, SAM)-competitive inhibitor of p rotein a rginine N- m ethyl t ransferases (PRMTs IC 50 = 8.81 &#181M for PRMT1 and 3.

Fast flow agarose comprises highly crosslinked agarose beads or gels that can tolerate high pressures compared to standard agarose bead resins. Thus, these resins are ideal for fast protein liquid chromatography (FPLC) purification methods.

Recombinant Protein G covalently bound to agarose by cyanogen bromide linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein G agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIP assays).

Protein G GraviTrap &#8482 includes ten ready-to-use columns prepacked with 1 mL of Protein G Sepharose &#8482 4 Fast Flow. Protein G GraviTrap &#8482 are prepacked gravity-flow columns designed for fast and efficient manual purification of

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification best for bovine, goat, human, and rat IgG and for mouse IgG 1 .

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.

A mixture of Protein G PLUS and Protein A covalently conjugated to agarose. Useful for purification of IgG from biological fluids.

Protein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.

Protein G was initially isolated from G148, a human group G Streptococcal strain. It is a cell wall protein and shows high affinity to IgG (immunoglobulin G).