PROTEIN G ALKALINE PHOSPHATASE CONJUGATE (C005B-229752)

General description

Protein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.

Protein G, Alkaline Phosphatase Conjugate is obtained by conjugation of protein G, a cell-surface protein of Streptococcus species to alkaline phosphatase. This conjugate is used as a secondary agent in immunoassay methods to detect polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a, and IgG2b.

Application

Protein G, Alkaline Phosphatase Conjugate has been used in:

• direct enzyme-linked immunosorbent assay (ELISA) (1:1000)(1:250)
• indirect ELISA (1:1000)
• immunoblot (1:5000)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.

Analysis Note

Single band at 33.8 kDa by SDS-PAGE

Other Notes

p-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl₂ at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

form: liquid. Quality Level: 100. contains: ≤. 0.1% sodium azide as preservative. manufacturer/tradename: Calbiochem^®. . storage condition: do not freeze. shipped in: wet ice. storage temp.: 2-8°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

41116127

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

500

Unit of Measurement:

UG

MPN:

539305-500UG

Temperature Control Device:

Yes