General description
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.
Application
Enzyme Immunoassay (1:1000, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, 10 mg/ml BSA.
Other Notes
Antibody should be titrated for optimal results in individual systems.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.01%
Human IgA: <0.01%
Human IgE: <0.01%
There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Hamilton, R.G. 1993. Clin. Chem.39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: mouse. Quality Level: 200. antibody form: purified antibody. antibody product type: secondary antibodies. clone: HP6043, monoclonal. form: liquid. does not contain: preservative. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 20°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 411550-500UG