MS pH Gradient Buffer B

pH gradient buffer is an innovative elution buffer (or mobile phase) that changes pH linearly for separation and purification of mAb antibodies, glycoproteins and PTM modified proteins by ion exchange (IEX) chromatography. In pH gradient buffer-based IEX chromatography, the pH of the initial buffer is kept at a constant level to ensure that the charged proteins bind to oppositely charged ions of the stationary phase. Then, the protein is eluted by linearly changing the pH of the buffer so that the protein has a zero net charge and can elute from the column. This buffer is suitable for IEX-MS to characterize intact mAb or subunit. Reagent Composition: Each bottle contains 0.3-1 g Ammonium acetate, and 0.2 g Organic acid. Resuspension: Dissolve reagents in 100 mL deionized, double-distilled water. pH Adjustment: At room temperature, slowly add LC grade ammonia adjust pH value to ensure the Buffer A pH=5.5; the Buffer B=8.5 for ready mobile phase of LC. Elution Buffer (mAb): pH Buffer A, pH 5.5 Elution Buffer B (mAb): pH Buffer B, pH 8.5 Linear pH Range: Linear correlation coefficient > 0.99 at pH 5.5 - 8.5 in columns of IEX chromatography. Reference Procedure for mAb subunit and intact protein: Column: SCX, 10 μm, 2.1 * 250 mm or 2.1 * 50 mm Flow rate: 0.3 mL/min Eluent A: 1X mAb pH buffer A (pH 5.5) Eluent B: 1X mAb pH Buffer B (pH 8.5) Gradient: 0 - 1 min, 0% B; 1 - 31 min, 0 - 100% B; 31 - 34 min, 100% B; 34 - 40 min, 0% B Temperature: 30 °C FLR detection: Ex: 280 nm, Em: 360 nm UV detection: 280 nm Load: 0.2 - 10 μg Inject volume: 4 μL

Specifications & Purity: pH gradient buffer is an innovative elution buffer (or mobile phase) that changes pH linearly for separation and purification of mAb antibodies, glycoproteins and PTM modified proteins by ion exchange (IEX) chromatography. In pH gradient buffer-based IEX chromatography, the pH of the initial buffer is kept at a constant level to ensure that the charged proteins bind to oppositely charged ions of the stationary phase. Then, the protein is eluted by linearly changing the pH of the buffer so that the protein has a zero net charge and can elute from the column. This buffer is suitable for IEX-MS to characterize intact mAb or subunit. Reagent Composition: Each bottle contains 0.3-1 g Ammonium acetate, and 0.2 g Organic acid. Resuspension: Dissolve reagents in 100 mL deionized, double-distilled water. pH Adjustment: At room temperature, slowly add LC grade ammonia adjust pH value to ensure the Buffer A pH=5.5; the Buffer B=8.5 for ready mobile phase of LC. Elution Buffer (mAb): pH Buffer A, pH 5.5 Elution Buffer B (mAb): pH Buffer B, pH 8.5 Linear pH Range: Linear correlation coefficient > 0.99 at pH 5.5 - 8.5 in columns of IEX chromatography. Reference Procedure for mAb subunit and intact protein: Column: SCX, 10 μm, 2.1 * 250 mm or 2.1 * 50 mm Flow rate: 0.3 mL/min Eluent A: 1X mAb pH buffer A (pH 5.5) Eluent B: 1X mAb pH Buffer B (pH 8.5) Gradient: 0 - 1 min, 0% B; 1 - 31 min, 0 - 100% B; 31 - 34 min, 100% B; 34 - 40 min, 0% B Temperature: 30 °C FLR detection: Ex: 280 nm, Em: 360 nm UV detection: 280 nm Load: 0.2 - 10 μg Inject volume: 4 μL

Grade: BioReagent

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

41115700

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

M1523267-1.50g

Country of Origin:

China

Product Size:

1.50g

Harmonized Tariff Code:

2931.90.90