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Sigma-Aldrich

MTT FORMAZAN (C005B-478107)

Application MTT Formazan has been used to measure cell viability. Biochem/physiol Actions MTT assay is a colorimetric reaction, generally used to examine cell viability.

Description

Application

MTT Formazan has been used to measure cell viability.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Specifications
UPC:
41115406
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
1
Unit of Measurement:
GM
MPN:
M2003-1G
CAS:
57360-69-7
Catalog No. C005B-478107
Price: $298.26
List Price: $331.40
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