N-Glycan Iabel

N-glycan label is used in ultra-fast labeling of glycans of PNGase F glycosidase-digested N-linked glycoproteins (including antibodies). Labeled glycan has highly sensitive fluorescence characteristics and ionization properties by mass spectrometry, allowing for quantitative analysis using LC-FLR and LC-MS. The unique chemical properties of this label provide higher fluorescence quantification and mass spectrometry response. Molecular Weight: 427.1850 Da Molecular Formula: C₂₁H₂₅N₅O₅ Mass Shift: + C₁₇H₂₀N₄O₂ = 312.3663 Da Labeled N-Glycan (Da) = Released N-Glycan + Glycan Label Reagent - Reaction by-product ( C₄H₅NO₃, MW: 115.0268) - H Resuspension: Dissolve 4.5 mg N-Glycan label in 55 μL freshly prepared DMF solution to reach 82 μg/μL. Application: 4.5 mg N-Glycan labeling reagent can label five digested glycan samples. Reference Procedure: Add 10 μL desalted 1.5 μg/μL mAb samples into 0.6 mL low protein binding collection tube. Add 10 μL 3% BT Surfactant with 5 mM TCEP and 50 mM HEPES (pH 7.9) to the tube and mix well. Heat at 95 °C for 3 min, then cool to the room temperature. Add 10 μL 1.5 U/μL rPNGase F and mix well. Incubated at 50 °C for 15 - 30 min, then cool to room temperature. Add 10 μL 82 μg/μL Glycan Label, incubate at room temperature for 5 min. Add 15 μL anhydrous DMF and 45 μL acetonitrile. Mix well. Labeled N-glycan samples can be used for LC-FLR and LC-MS analysis.

Specifications & Purity: N-glycan label is used in ultra-fast labeling of glycans of PNGase F glycosidase-digested N-linked glycoproteins (including antibodies). Labeled glycan has highly sensitive fluorescence characteristics and ionization properties by mass spectrometry, allowing for quantitative analysis using LC-FLR and LC-MS. The unique chemical properties of this label provide higher fluorescence quantification and mass spectrometry response. Molecular Weight: 427.1850 Da Molecular Formula: C₂₁H₂₅N₅O₅ Mass Shift: + C₁₇H₂₀N₄O₂ = 312.3663 Da Labeled N-Glycan (Da) = Released N-Glycan + Glycan Label Reagent - Reaction by-product ( C₄H₅NO₃, MW: 115.0268) - H Resuspension: Dissolve 4.5 mg N-Glycan label in 55 μL freshly prepared DMF solution to reach 82 μg/μL. Application: 4.5 mg N-Glycan labeling reagent can label five digested glycan samples. Reference Procedure: Add 10 μL desalted 1.5 μg/μL mAb samples into 0.6 mL low protein binding collection tube. Add 10 μL 3% BT Surfactant with 5 mM TCEP and 50 mM HEPES (pH 7.9) to the tube and mix well. Heat at 95 °C for 3 min, then cool to the room temperature. Add 10 μL 1.5 U/μL rPNGase F and mix well. Incubated at 50 °C for 15 - 30 min, then cool to room temperature. Add 10 μL 82 μg/μL Glycan Label, incubate at room temperature for 5 min. Add 15 μL anhydrous DMF and 45 μL acetonitrile. Mix well. Labeled N-glycan samples can be used for LC-FLR and LC-MS analysis.

Grade: BioReagent;Suitable for mass spectrometry (MS)

Molecular Formula: C21H25N5O5

Molecular Weight: 427.45

PubChem CID: 57358310

Isomeric SMILES: C[C@@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-])N

Storage Temperature: Store at -20°C

Shipped in: Ice chest + Ice pads

UPC:

51171518

Weight:

1.09 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

N1523263-4.5mg

Country of Origin:

China

CAS:

61043-41-2

Product Size:

4.5mg

Harmonized Tariff Code:

2931.90.90