BIOWORLD
Ni-Chelated Separoporer (Agarose)4B,50mL
Ni-Chelated Separopore® 4B is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Imminodiacetate immobilized matrix charged with
Ni-Chelated Separopore® 4B is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Imminodiacetate immobilized matrix charged with Ni metal ions. IIdeal for purification of recombinant proteins containing (His)6 fusion tag. Technical Specifications Chelating group: Imminodiacetic acid Chelating group coupling method: epoxy activation Matrix: Cross-linked Separopore® 4% agarose beads Particle size (range): 50-168m Metal ion capacity: > 20mol Ni2+/ml gel pH stability: 2-11 Binding Capacity: Approx. 5-8 mg histidine-tagged protein/ml medium Chemical Stability For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1 M NaOH, 70% acetic acid 30 min tested: 30% 2-propanol 1 h tested: 2% SDS Compatibility during use Stable in all commonly used buffers, reducing agents, denaturants, and detergents Supplied as suspension in 20% ethanol. Storage and shipping: Shipped at ambient condition, refrigerate upon reciept. Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemica
Specifications
- UPC:
- 41106510
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- Quantity:
- 1
- Unit of Measurement:
- EA
- WeightUOM:
- LB
- MPN:
- 20181046-3



