NS0-TURBODOMA

Cell Line Origin

Mouse myeloma, serum-free, without cholesterol

Cell Line Description

NS0 cell line (Sigma Catalogue no. 85110503) adapted to grow in the chemically defined protein- and peptide-free medium Turbodoma (Cell Culture Technologies). Cholesterol is not required as a supplement for this culture. This cell line is suitable for use as a fusion parent for serum-free generation of hybridomas. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.

Culture Medium

Turbodoma medium (Cell Culture Technologies) + 2mM L-Glutamine (G7513). When freezing cells down use TurboDoma^™ medium (50:50 conditioned medium:fresh medium) + 2mM L-Glutamine (G7513) + 10% DMSO.

Subculture Routine

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Legal Information

TurboDoma is a trademark of Cell Culture Technologies

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Shipping Information:

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UPC:

41106514

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VL

MPN:

06020202-1VL

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes