Sigma-Aldrich
O-PHENYLENEDIAMINE DIHYDROCHLORIDE (C005B-440387)
General description o -Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble
Description
General description
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
Application
o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in enzyme-linked immunosorbent assay (ELISA) procedures.
Caution
May darken in storage.
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Specifications
- UPC:
- 51372127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- Yes
- Quantity:
- 100
- Unit of Measurement:
- GM
- MPN:
- P1526-100G
- CAS:
- 615-28-1
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