OCTADECYL-FUNCTIONALIZED SILICA GEL 22-24% CARBON LOADING

General description

Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.

Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.

Warning

Toxicity: Standard Handling (A)

Physical form

150 mM NaCl, 100 mM Tris-HCl, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Li, Y., et al. 2002. Biochemistry41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160.
Anderson, O.M., et al. 2001. Biochemistry40, 15408.
Bu, G. 2001. Int. Rev. Cytol.209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

UPC:

12352211

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

50

Unit of Measurement:

UG

MPN:

553506-50UG