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Sigma-Aldrich

OPTIPREP DENSITY GRADIENT MEDIUM USED FOR CELL AND SUBCELLULAR ORGANELLE ISOLATION (C005B-090308)

OptiPrep ™ Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in

Description

General description

OptiPrep Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in vitro isolation of biological particles. It may be used for fractionation and separation of cell and subcellular organelles.

Application

OptiPrep has been used to isolate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells. It has also been used for the following applications:

  • to investigate the efficiency of single-cell encapsulation.
  • the gradient-based isolation of senescent cancer cells.
  • isolation of non-enveloped virion isolation from bronchoalveolar lavage.
  • isolation of lipoproteins and macromolecules
  • purification of virions
  • purification of neuron cells

Features and Benefits

OptiPrep has numerous biocompatible properties that make it ideal for use.

  • It is biologically inert and does not interfere with assays for marker enzymes.
  • It is non-ionic, non-toxic, and has low osmotic pressure.
  • It forms spontaneous gradients that are stabler and easier to prepare than sucrose.

Other Notes

60% (w/v) solution of iodixanol in water (sterile).

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Specifications
UPC:
41115310
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
250
Unit of Measurement:
ML
MPN:
D1556-250ML
CAS:
92339-11-2
Catalog No. C005B-090308
Price: $526.62
List Price: $585.13
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