P22 HAMSTER CHINESE OVARY (C005B-235383)

Cell Line Origin

Hamster Chinese ovary

Cell Line Description

Chinese hamster ovary cell line which lacks ornithine decarboxylase activity.

Culture Medium

EMEM (EBSS) + Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.1% BSA.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.1% cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 min. or until cells detach; 5% CO₂; 37°C. Pre-treat culture flasks with porcine gela

Other Notes

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biological source: hamster ovary. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

51111850

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VI

MPN:

89030317-1VL

Temperature Control Device:

Yes